PHA-767491抑制CDC-7的表达在子宫内膜癌ishikawa细胞系中的作用研究

目的:PHA-767491抑制CDC-7的表达对子宫内膜癌ishikawa细胞系的增殖、侵袭、迁移以及凋亡产生的作用。方法:配置PHA-767491至各实验所需浓度，使其作用于子宫内膜癌ishikawa细胞系。CCK-8法用于检测ishikawa细胞系的增殖能力及明确IC50。运用qRT-PCR法，检测药物PHA-767491对ishikawa细胞中的CDC-7 mRNA的表达的影响。利用划痕实验，检测PHA-767491对于ishikawa细胞迁移能力的影响。用Transwell法检测药物影响ishikawa细胞的侵袭能力。流式细胞术Annexin V/PI双染色法检测药物PHA-767491影响ishikawa细胞的凋亡能力。结果:CCK-8的结果得出，药物PHA-767491能够有效地抑制ishikawa细胞系增殖的能力，得出24 h药物IC50等于9.51 μmol/L。qRT-PCR的结果证明：PHA-767491抑制ishikawa细胞中CDC-7 mRNA的显著表达。划痕实验的结果表明：PHA-767491可抑制ishikawa细胞的迁移能力。Transwell结果表明：药物PHA-767491有效地抑制ishikawa细胞的侵袭能力。流式细胞术结果证明：PHA-767491可以促进ishikawa细胞的凋亡。结论:PHA-767491能够显著抑制子宫内膜癌细胞ishikawa中CDC-7的表达，降低了ishikawa细胞增殖和迁移、侵袭能力，并且可明确诱导ishikawa细胞的凋亡。

Effects of PHA-767491 on the inhibition of CDC-7 expression in endometrial cancer ishikawa cell line

AIM: To investigate the effects of PHA-767491 on the inhibition of CDC7 expression on proliferation, invasion, migration and apoptosis of endometrial cancer ishikawa cell line. METHODS: Different concentrations of PHA-767491 were used to treat the endometrial cancer ishikawa cell line. The proliferation ability of ishikawa cells and the IC50 were determined by CCK-8 method. RT-PCR was used to detect the effect of PHA-767491 on the expression of CDC-7 mRNA in ishikawa cells. The migration ability of the cells was then examined using a scratch test. The transwell chamber method was applied to detect the invasive ability of ishikawa cells.Flow cytometry Annexin V/PI double staining was used to detect cell apoptosis. RESULTS:The results of CCK-8 assay showed that PHA-767491 significantly inhibited the proliferation of endometrial cancer ishikwa cells with an IC50 of 9.51 μmol/L at 24 hours. RT-PCR showed that PHA-767491 significantly inhibited the expression of Cdc-7 mRNA in ishikawa cells. Scratch test showed that PHA-767491 effectively inhibited the migration ability of ishikawa cells. Transwell chamber results showed that PHA-767491 inhibited the invasion of ishikawa cells.PHA-767491 significantly induced ishikawa cells apoptosis.CONCLUSION: PHA-767491 can significantly inhibit the expression of CDC-7 in ishikawa of endometrial cancer cells, which can significantly reduce the proliferation, migration and invasion and induce apoptosis of ishikawa cells.