胡桃醌对人卵巢癌SKOV3和IOSE80细胞增殖和侵袭的抑制作用及其机制研究

目的: 研究胡桃醌对人卵巢癌SKOV3和IOSE80细胞增殖和侵袭的抑制作用，并从信号通路深入探讨其作用机制。方法: 体外培养人卵巢癌SKOV3细胞和IOSE80细胞，给予不同浓度的胡桃醌溶液干预，MTT比色法检测细胞增殖，划痕实验检测细胞迁移，Transwell检测细胞转移，流式细胞术检测细胞凋亡，Western Blot检测AKT、ERK、NF-κB信号通路蛋白表达，RT-PCR检测波形蛋白(vimentin)、基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）mRNA表达。 结果: 不同浓度胡桃醌可抑制SKOV3细胞和IOSE80细胞增殖活力，降低细胞侵袭能力，加速细胞凋亡，且随浓度的增加对细胞增殖、侵袭和凋亡的作用越明显，差异均有统计学意义（P<0.05）。不同浓度胡桃醌均可抑制SKOV3细胞和IOSE80细胞AKT、ERK、NF-κB信号通路蛋白的表达，且随浓度的增加信号通路蛋白表达减少，差异均有统计学意义（P<0.05）。不同浓度胡桃醌均可减少SKOV3细胞和IOSE80细胞Vimentin、MMP-2、MMP-9 mRNA的表达，且随浓度的增加mRNA表达减少，差异均有统计学意义（P<0.05）。结论: 胡桃醌可抑制SKOV3和IOSE80细胞体外增殖、转移，促进凋亡，其机制与抑制AKT、ERK、NF-κB信号通路表达和下调Vimentin、MMP-2、MMP-9 mRNA表达有关。

Inhibitory effect of juglone on proliferation and invasion of human ovarian cancer SKOV3 and IOSE80 cells and its mechanism

AIM: To study the effect of juglone on proliferation and invasion of human ovarian cancer SKOV3 and IOSE80 cells, and to explore its mechanism through signal pathway. METHODS: Cells were cultured in vitro and treated with different concentrations of juglone. The cell proliferation was detected by MTT colorimetry, the cell migration was detected by scratch test, the cell metastasis was detected by Transwell, and the apoptosis was detected by flow cytometry. The protein expression of AKT, ERK, NF-κB signaling pathway were detected by Western blot, and the expressions of Vimentin, MMP-2 and MMP-9 mRNA were detected by RT-PCR. RESULTS: Different concentrations of juglone could inhibit the proliferation of SKOV3 and IOSE80 cells, reduce the invasive ability of cells, and accelerate the apoptosis, with increase of concentration the effect on cell proliferation, invasion and apoptosis were more obvious, the difference was statistically significant (P<0.05). The expressions of AKT, ERK and NF-κB signaling pathway proteins in SKOV3 and IOSE80 cells were decreased by different concentrations of juglone, and the expression decreased with the increase of concentration, the difference was statistically significant (P<0.05). The expressions of Vimentin, MMP-2 and MMP-9 mRNA in SKOV3 and IOSE80 cells were decreased with the increase of concentration of juglone, and the difference was statistically significant (P<0.05). CONCLUSION: Juglone can inhibit the proliferation and metastasis of SKOV3 and IOSE80 cells in vitro and promote apoptosis. The mechanism maybe related to inhibit the expression of AKT, ERK, NF-κB signaling pathway, downregulate of the expression of Vimentin, MMP-2 and MMP-9 mRNA.