Spinmarkierte Oligopeptide als substratanaloge Inhibitoren der Leucinaminopeptidase

Spin Labeled Oligopeptides as Substrate Analogous Inhibitors of Leucine Aminopeptidase The synthesis of L-Thr(Bu^t)-(L-Phe)_n-SL peptides C-terminal labeled with the nitroxide spin label 2,2,5,5-tetramethyl-3-amino-pyrrolidin-1-oxyl, is described and e.s.r. parameters of the labeled compounds are presented. As shown by e.s.r. spectroscopy studies Thr(Bu^t)-Phe-SL is a competitive inhibitor of the leucine aminopeptidase and is bound by the enzyme with high affinity.