酿酒功能菌的基因组测序及基因功能注释

为挖掘库德毕赤酵母FJZ在清香型白酒酿造过程中的代谢机制，基于Illumina Novaseq和PacBio测序平台对菌株FJZ进行基因组测序，将测序数据在碳水化合物活性酶(CAZy)数据库进行比对，并通过GO、KOG和KEGG数据库进行基因功能注释。CAZy分析表明:菌株FJZ具有催化酚类化合物转化合成芳香化合物的香草醇氧化酶(VAO)活性，具有羧酸酯酶催化合成和分解乙酸乙酯、乳酸乙酯的能力，具有甘露糖转移酶催化甘露糖转移到底物生成多萜寡糖的前体的能力等。在GO数据库共注释分子功能类别的基因6 320个，具有转移烷基或芳基、糖基、酰基等与酯合成相关的转移酶活性。在KEGG的7类功能数据库中共注释基因7 815个，其中代谢相关的基因有1 406个，萜类和聚酮类物质代谢相关的基因30个。在KOG共注释到基因3 704个，辅酶运输和代谢相关的基因83个等。本研究为清香型白酒酿造过程中酶系的研究，以及白酒风味物质形成机理和酒醅功能菌结构探索提供参考依据。

Genome Sequencing Analysis and Gene Function Annotations of Functional Brewing Fungi

In order to explore the metabolic mechanism of Pichia kudriavzevii FJZ in the brewing process of light-aroma Baijiu， the genome of the strain FJZ was sequenced based on Illumina Novaseq and PacBio sequencing platform， and the sequencing data was compared and analyzed in the carbohydrate active enzyme (CAZy) database， gene function annotation was also performed in GO， KOG and KEGG databases. In the CAZy analysis， it was shown that the strain FJZ had the activity of vanillyl alcohol oxidase(VAO) that catalyzed the conversion of phenolic compounds into aromatic compounds， carboxylesterase catalyzed the synthesis and decomposition of ethyl acetate and ethyl lactate， it had α-1，2-mannose transferase to catalyze mannose to form a precursor to polyterpenoid oligosaccharides. In the GO database， 6 320 genes with molecular function was annotated， it included transferase activities related to ester synthesis， they had the function of transferring alkyl or aryl groups， sugar groups， acyl groups， etc. In the KEGG''s seven functional database， a total of 7 815 genes were annotated， of which 1 406 genes were metabolic-related genes， and 30 genes were included terpenoids and polyketides. KOG annotated a total of 3 704 genes， coenzyme transport and metabolism 83 genes. This study provides a basis for the study of enzyme lines in the brewing process of light-aroma baijiu， as well as the formation mechanism of liquor flavor substances and the structure of functional bacteria of liquor.