Development of an electrochemical‐surface plasmon dual biosensor based on carboxylated conducting polymer thin films |
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| Authors: | Saengrawee Sriwichai Rapiphun Janmanee Sukon Phanichphant Kazunari Shinbo Keizo Kato Futao Kaneko Tadashi Yamamoto Akira Baba |
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| Affiliation: | 1. Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand;2. Materials Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand;3. Department of Chemistry, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok, Thailand;4. Graduate School of Science and Technology and Center for Transdisciplinary Research, Niigata University, Niigata, Japan;5. COI‐s Biofluid Biomarker Center, Institute for Research Collaboration and Promotion, Niigata University, Niigata, Japan |
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| Abstract: | A biosensing platform based on the covalent attachment of biomolecules on electropolymerized carboxylated conducting polymers, poly(3‐aminobenzoic acid) and poly(3‐pyrrole carboxylic acid), were developed for the selective simultaneous detection of two biomolecules using electrochemical‐surface plasmon resonance (EC–SPR) spectroscopy. The surface morphology of the developed biosensors was studied by scanning electron microscopy and atomic force microscopy. The EC–SPR dual biosensor was developed for the label‐free, simultaneous, and selective detection of glucose and human immunoglobulin G (IgG). A change in current density was clearly observed after the injection of glucose, whereas a change in SPR reflectivity was clearly observed after the injection of human IgG. The present work demonstrates the potential of this biosensing platform for real sample analysis in the future. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135, 45641. |
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| Keywords: | biosensor conducting polymer electrochemical‐surface plasmon resonance spectroscopy glucose human immunoglobulin G |
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