Fabrication and in vitro characterization of antibacterial magneto-luminescent core-shell bioactive glass nanoparticles |
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| Affiliation: | 1. Department of Metallurgical and Materials Engineering, Hitit University, Corum, Turkey;2. Department of Metallurgical and Materials Engineering, Middle East Technical University, Ankara, 06800, Turkey;3. BIOMATEN, Center of Excellence in Biomaterials and Tissue Engineering, Middle East Technical University, 06800, Ankara, Turkey;4. Biomedical Engineering Program, Middle East Technical University, Ankara, Turkey;1. Spectroscopy Department, Physics Research Institute, National Research Center, 12311, 33 Elbehouth st., Dokki, Cairo, Egypt;2. Department of Physics, Faculty of Science, Mansura University, Mansoura, 35516, Egypt;1. Materials Science & Engineering, Texas A&M University, College Station, TX, United States;2. Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson AFB, OH, United States;1. State Key Laboratory of Advanced Welding and Joining, Harbin Institute of Technology, Harbin, 150001, China;2. Zhengzhou Research Institute, Harbin Institute of Technology, Zhengzhou, 450018, China;3. Key Laboratory of Advanced Structural-Functional Integration Materials & Green Manufacturing Technology, Harbin Institute of Technology, Harbin, 150001, China;4. National Key Laboratory of Science and Technology on Tunable Laser, Harbin Institute of Technology, Harbin, 150001, China;1. School of Medicine, Anhui University of Science and Technology, Huainan, PR China;2. Center for Orthopaedic Science and Translational Medicine, Department of Orthopaedics, Shanghai Tenth People''s Hospital, School of Medicine, Tongji University, 301 Yanchang Road, Shanghai, 200072, PR China |
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| Abstract: | When nanomaterials with antibacterial properties were sent to the infected area, it was predicted that infection and related complications could be prevented. The nanoparticles can be designed to possess magnetic and luminescence (magneto-luminescent) properties to be effectively targeted and localized at the infection foci without dispersing into the body. Simultaneously, the magneto-luminescent characteristic of particles allows visualization and confirmation of localized particles at the desired area. In this regard, there are no studies on the use of antibacterial magneto-luminescent bioactive glass for orthopedic applications and the treatment of orthopedic device-related infections. In this study, antibacterial magneto-luminescent 58S bioactive glasses were synthesized by the modified Stöber using coupled with a layer-by-layer assembly approach to possess core/shell particle morphology. SPION/Bioactive glass nanoparticles had an average size of 50 nm and displayed superparamagnetic behavior. While the saturation magnetization value (σs) of the undoped 58S sample was 25.32 emu/g, that of the co-doped sample (2% Eu, 2% Zn) was 21.74 emu/g; this showed that the doping slightly reduced the magnetization value. Europium (Eu) doping of SPION/Bioactive glass nanoparticles induced characteristic red emission originating from Eu emissions belonging to 5D0–7FJ (J = 1–4) transitions and the strongest peak was at 612 nm (electric-dipole transition, 5D0–7F2). Color chromaticity coordinates confirmed emission in the red region. XPS spectrum revealed the existence of Eu and Zn dopant elements in 58S bioactive glass. After soaking characteristic peaks at 31.74° and 45.43° belonging to the hexagonal hydroxyapatite phase were detected in the XRD data, confirming the SEM images. 2% Eu doped SPION/Bioactive glass nanoparticles had the highest osteoblast viability up to 7 days in vitro, while doping the samples with 2% zinc did not yield bone cell viability as high as the Eu doped ones. Importantly, Eu doped SPION/Bioactive glass nanoparticles inhibited gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli) growth up to 48 h in vitro. The results showed that Eu doping of SPION/Bioactive glass nanoparticles increased osteoblast viability and inhibited bacterial growth, while possessing superparamagnetic properties and exhibiting red luminescence. |
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| Keywords: | Bioactive glass Core/shell nanoparticles Antibacterial Luminescence Magnetic Europium |
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