Hepatic bile acid elution by albumin and bile acid content in isolated rat hepatocytes |
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Authors: | Shigeru Hashimoto Kiyohisa Uchida Masaharu Hirata |
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Affiliation: | (1) Shionogi Research Laboratories, Shionogi & Co., Ltd., Fukushima-ku, 553 Osaka, Japan |
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Abstract: | Bile acid contents were determined for isolated rat hepatocytes. During the course of isolating the hepatocytes, perfusion
of rat liver with buffer containing 2% albumin eluted a significant amount of bile acids. The elution was proportional to
the volume of the buffer and attributable to albumin in the buffer. The isolated hepatocytes prepared by perfusion with 0.1%
albumin buffer, which eluted a negligible amount of bile acids, contained 95±12 μg/108 cells of bile acids. The major bile acids were cholic acid (22%), β-muricholic acid (34%) and hyodeoxycholic acid (10%).
Levels of the other bile acids were less than 3%. Peak 8, unidentified but presumed to be a trihydroxycholanoic acid, accounted
for 19%. |
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Keywords: | |
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