南美白对虾养殖水体5株疑似病原菌的分离与初步鉴定

应用TCBS(硫柠胆蔗琼脂)培养基从上海金山区南美白对虾(Litopenaeus vannamei)养殖水体中分离筛选出5株优势疑似病原菌(编号为S1、S2、S3、S4、S5),应用ARDRA图谱分析、ERIC-PCR分析结合16S rD- NA的PCR扩增与测序等多相分子生物学方法对其进行了鉴定。结果表明,S1为Pseudomonas属细菌;S2为Leclercia属细菌;S3为Exihuobacterium属细菌;S4为Jeotgalibacillus属细菌;S5为Staphylococcus属细菌。GenBank登录号为EU251039～EU251043。其中S2、S3、S4是首次从南美白对虾养殖水体中分离出来的疑似病原菌。结果还表明,TCBS培养基并非专一性选择培养弧菌的培养基,其他疑似病原菌也可以在该培养基中生长。

Isolation and Preliminary Identification of Five Suspicious Pathogenic Bacteria from Aquatic Water of White-leg Shrimp Litopenaeus vannamei

In this study five suspicious pathogenic bacterial strains(No.S1,S2,S3,S4 and S5)were iso- lated from the aquatic water of white-leg shrimp Litopenaeus vannamei in Jinshan district of Shanghai with TCBS culture medium.Preliminary identifications of these strains by ARDRA(amplified ribosomal DNA re- striction analysis),ERIC-PCR(enterobacterial repetitive intergenic consensus-Polymerase Chain Reaction, PCR)and sequencing of 16S rDNA were done.The preliminary results showed that S1 was Pseudomonas,S2 was Leclercia,S3 was Exihuobacterium,S4 was Jeotgalibacillus,S5 was Staphylococcus.GenBank acces- sion for these five strains are EU251039～EU251043.It was the first time that the suspicious pathogenic bac- teria of S2,S3 and S4 were isolated from aquatic water of white-leg shrimp Litopenaeus vannamei.The re- sults also showed that TCBS culture medium was not the specific selective culture medium just for Vibrio.It was useful for healthy aquaculture of white-leg shrimp Litopenaeus vannamei.