Cultivable bacterial diversity and amylase production in three typical Daqus of Chinese spirits |
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Authors: | Zuming Li Zhihui Bai Deliang Wang Wujiu Zhang Michael Zhang Francis Lin Liping Gao Bodi Hui Hongxun Zhang |
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Affiliation: | 1. College of Arts and Sciences of Beijing Union University, , Beijing, 100191 China;2. Research Center for Eco‐Environmental Sciences, Chinese Academy of Sciences, , Beijing, 100085 China;3. China National Research Institute of Food and Fermentation Industries, , Beijing, 100027 China;4. Department of Physics and Astronomy, University of Manitoba, , Winnipeg, MB, R3T 2N2 Canada |
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Abstract: | Both culture‐dependent method and molecular technique were firstly used to simultaneously investigate the cultivable bacterial diversity and amylase production in three typical Daqus of Chinese spirits. The results showed that both cultivable bacterial diversity and amylase production were obviously different. The species of nine bacteria from Deshan, nine from Baisha and six from Wuling Daqus were identified. The total bacterial strains of 17, 15 and 14, and 9, 16 and 10 could produce α‐amylase and glucoamylase, respectively, from the Daqus, and the enzyme yields were different. Bacillus licheniformis, Bacillus subtilis and Bacillus amyloliquefaciens not only were dominant bacteria in the Daqus, but also possessed high activities of α‐amylase and glucoamylase. By comparison, Bacillus cereus and Bacillus oleronius were found to be another predominant bacterial species and good producers of α‐amylase and glucoamylase in Deshan and Wuling Daqus, respectively. |
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Keywords: | 16S rRNA gene bacterial diversity Daqu glucoamylase α ‐amylase |
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