| 非发酵豆制品中合成着色剂测定方法的研究 |
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| 引用本文： | 王鑫,黄韬睿.非发酵豆制品中合成着色剂测定方法的研究[J].生命科学仪器,2012,10(5):26-27. |
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| 作者姓名： | 王鑫 黄韬睿 |
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| 作者单位： | 1. 四川省食品药品检验所,成都,610097
2. 四川烹饪高等专科学校,成都,610100 |
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| 摘 要： | 建立了非发酵豆制品中合成着色剂测得的高效液相色谱法。用无水乙醇-氨水（2ml浓氨水稀释至100m1）-水（70：20：10）提取合成着色剂，采用AgilentEclipseXDB—C18（4．6ram×150mm，5μm）；流动相：甲醇-0．02m0儿乙酸铵，梯度洗脱，流速为1．0ml／min，柱温：35℃，检测波长：254nm。在此色谱条件下进行检测，结果表明，两种合成着色剂，方法回收率均为90％以上，方法最低检出限均为0．02mg／kg。该方法可有效检测非发酵豆制品中合成着色剂含量。
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| 关 键 词： | 非发酵豆制品 合成着色剂 高效液相色谱 |
Determination of Synthetic Colorants in Non-Fermented Bean Products |
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| Xin Wang,Taorui Huang.Determination of Synthetic Colorants in Non-Fermented Bean Products[J].Life Science Instruments,2012,10(5):26-27. |
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| Authors: | Xin Wang Taorui Huang |
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| Affiliation: | 1.Sichuan Institute For Food and Drug Control, Chengdu 610097, Sichuan, China; 2. Sichuan Higher Institute of Cuisine, Chengdu 610100, Sichuan, China) |
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| Abstract: | Establish a method to determination of synthetic colorants in non-fermentedbean products by HPLC. extraction of synthetic colorants with Anhydrous ethanol - ammonia.Use Agilent Eclipse XDB-C18 （4.6mm ×150mm, 5 μ m ） ; Mobile phase： methanol-0.02mol / L ammonium acetate; Gradient elution with a flow rate of 1.0ml/min, column temperature： 35℃, detection wavelength： 254nm. Be detected under this chromatographic conditions, results show that the method recoveries were 90%, method minimum detection limits were 0.02mg/kg. This method can effectively detect the content of synthetic colorants in non-fermented bean products. |
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| Keywords: | Bean products Synthetic Ccolorants HPLC |
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