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荔枝壳中多酚氧化酶活性研究
引用本文:熊何健,卢玉兰. 荔枝壳中多酚氧化酶活性研究[J]. 食品科学, 2006, 27(12): 182-185
作者姓名:熊何健  卢玉兰
作者单位:集美大学生物工程学院;
摘    要:本文对荔枝壳中多酚氧化酶(PPO)的活性及不同抑制剂的抑制效果进行研究。结果表明:荔枝壳多酚氧化酶的最佳底物浓度为0.04mol/L,最适pH为7.0,最适温度为40℃;柠檬酸、抗坏血酸、硫代硫酸钠对PPO活性表现出较好的抑制效果,三种抑制剂联合作用的最佳条件为0.01mmol/L的柠檬酸+0.04mmol/L的抗坏血酸+0.004mmol/L的硫代硫酸钠,PPO活性的抑制率达85.8%。

关 键 词:荔枝壳   多酚氧化酶   活性   抑制  
文章编号:1002-6630(2006)12-0182-04
收稿时间:2006-08-10
修稿时间:2006-08-10

Characteristics of Polyphenol Oxidase in Lichi Hull
XIONG He-jian,LU Yu-lan. Characteristics of Polyphenol Oxidase in Lichi Hull[J]. Food Science, 2006, 27(12): 182-185
Authors:XIONG He-jian  LU Yu-lan
Affiliation:School of Bioengineering, Jimei University, Xiamen 361021, China
Abstract:The activity of PPO of lichi hull and the control effect for different inhibitor were studied in this paper. The result showed that the fine substrate consistence was 0.04mol/L, the proper pH was 7.0, the optimum temperature was 40℃ for PPO of lichi hull;citric acid, ascorbic acid,hyposulphite display better inhibition effect on PPO activity. The optimum inhibition condition was 0.05mmol/L citric acid add 0.2mmol/L ascorbic acid add 0.02mmol/L hyposulphite, the PPO residual activity was 14.2%.
Keywords:lichi hull   polyphenol oxidase   activity   inhibition
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