| 藤黄微球菌过氧化氢酶基因在大肠杆菌中的重组与表达 |
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| 引用本文： | 尚柯,郑宪友,陈婷,疏翠,曹张军,张兴群. 藤黄微球菌过氧化氢酶基因在大肠杆菌中的重组与表达[J]. 山东食品科技, 2012, 0(2): 81-84 |
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| 作者姓名： | 尚柯 郑宪友 陈婷 疏翠 曹张军 张兴群 |
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| 作者单位： | [1]东华大学／江南大学生态纺织教育部重点实验室,上海201620 [2]上海交通大学附属第六人民医院骨科,上海200233 [3]东华大学化学化工与生物工程学院,上海201620 |
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| 基金项目： | 上海市科委纳米技术专项（1052nm05702） |
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| 摘 要： | 目的应用基因重组技术在大肠杆菌中高效表达藤黄微球菌过氧化氢酶（catalase，CAT）。方法从藤黄微球菌DNA中获得CAT编码基因，并应用pProEx．HTa质粒构建融合表达载体并表达和检测活性。结果通过融合表达获得可溶性带6×His标签重组蛋白，该蛋白经过Ni-NTA纯化后可获得活性物质。结论从大肠杆菌表达体系中表达了具有生物活性的CAT。
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| 关 键 词： | 过氧化氢酶基因：大肠杆菌 克隆 表达 |
Recombination and Expression of Micrococcus luteus Catalase in E. coli |
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| SHANG Ke,ZHENG Xian-you,CHEN Ting,SHU Cui,CAO Zhang-jun,ZHANG Xing-qun. Recombination and Expression of Micrococcus luteus Catalase in E. coli[J]. , 2012, 0(2): 81-84 |
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| Authors: | SHANG Ke ZHENG Xian-you CHEN Ting SHU Cui CAO Zhang-jun ZHANG Xing-qun |
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| Affiliation: | 1. Donghua University/Jiangnan University, Key Laboratory of Science ＆ Technology of Eco-Textile Ministry of Education, Shanghai 201620, China; 2. Department of Orthopedic Surgery, Shanghai No.6 Hospital affiliated to Shanghai Jiaotong University, Shanghai 200233, China; 3. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China) |
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| Abstract: | Objective To produce Micrococcus luteus catalase （CAT） on a large scale in E. coli by recombinant DNA technology. Methods CAT gene was obtained by PCR from Micrococcus luteus DNA, and the expression vectors were constructed by using pProEx-HTa plasmids and transformed into E. coli. Results The fusion expressed recombination proteins were soluble and labeled with 6 × His. By Ni-NTA affinity chromatography, the active CAT was obtained from the recombination proteins. Conclusion The recombinant Micrococcus luteus CAT with biological activity can be obtained from E. coli expression system. |
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| Keywords: | catalase gene E. coli cloning expression |
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