| HPLC测定保健食品中黄芪甲苷的含量 |
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| 引用本文： | 唐炜,李学涛. HPLC测定保健食品中黄芪甲苷的含量[J]. 山东食品科技, 2012, 0(2): 120-122 |
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| 作者姓名： | 唐炜 李学涛 |
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| 作者单位： | [1]临沂市产品质量监督检验所,山东临沂276001 [2]辽宁中医药大学药学院,辽宁大连116600 |
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| 摘 要： | 目的建立以高效液相色谱法测定保健食品中黄芪甲苷含量的方法。方法色谱柱Gemini C18（4．6mm×250nm，5μm），流动相乙腈-水（32：68），流速1．0mL／min，检测波长205nm，柱温30℃，进样量20此。结果黄芪甲苷检测浓度的线性范围0．2～8．0μg（r=0．9999）；平均加样回收率99．35％（RSD=0．79％）。结论本方法简便、准确、重现性好，可用于测定蜂胶黄芪软胶囊中黄芪甲苷的含量。
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| 关 键 词： | 蜂胶黄芪软胶囊 高效液相色谱法 黄芪甲苷 |
Determination of Astragaloside IV in Health Food by HPLC |
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| TANG Wei,LI Xue-tao. Determination of Astragaloside IV in Health Food by HPLC[J]. , 2012, 0(2): 120-122 |
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| Authors: | TANG Wei LI Xue-tao |
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| Affiliation: | 1. Linyi Institute of Supervision ＆ Testing on Product Quality, Linyi 276001, China; 2. School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China) |
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| Abstract: | Objective To establish a HPLC method for the determination ofastragaloside IV in health food. Methods The experiment was carried out on Kromasil C18 （4.6 mm × 250 mm, 5 μm）column with the mobile phase consisting of acetonitrile-water（32 ： 68） at a flow rate of 1.0 mL.min1. The detection wavelength was 205 nm and the column temperature was 30℃. The sample size was 20 μL. Results The linear range of astragaloside IV was 0.2-8.0 μg （r = 0.999 9）. The average recovery was 99.35 % （RSD =0.79 %）. Conclusion The method employed in this analysis is simple, precise and reproducible, and it can be used for the determination of astragaloside IV in propolis astragalus soft capsule. |
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| Keywords: | propolis astragalus soft capsule HPLC astragaloside IV |
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