梨果棒曲霉素产生菌的分离及ITS区鉴定

以莱阳仕梨、阳信鸭梨腐败果为材料,分离棒曲霉素产生菌并对其进行形态及ITS区鉴定。试验采用高效液相色谱法(HPLC)检测棒曲霉素含量,确定所分离菌株产毒能力,通过对产毒菌株保守区域ITS扩增测序,得到的序列号与GenBank中核酸数据库进行对比,初步确定了各菌株的亲缘菌,建立系统发育树。结果表明:分离得到10株不同能力的棒曲霉素产生菌,7株为青霉属,2株为曲霉属,1株为镰刀菌属;进化发育关系为:P-9菌为一类群,其他9株菌为另一类群,彼此之间存在不同程度的进化关系。易污染梨果的产毒霉菌主要为青霉菌,分离得到P-1菌株的产毒能力最强。

Isolation of Main Patulin-producing Strains from Storage Pears and Their ITS Sequences Analysis and Identification

This study isolated main patulin-producing strains from decayed Yang Xin and Lai Yang pears,analyzed and identified their morphological characteristics and ITS sequences.In this paper,patulin-producing strains were isolated from storage pears and test the concentration of patulin produced by HPLC.The fungal ITS region-specific primers were used to do ITS PCR amplification sequencing,through the blast analysis between ITS sequences and GenBank nucleic acid database,finally their biological classifications were identified and their relationships were analyzed.Result: 10 patulin-producing strains were isolated from storage pears,including 1 strains of Fusarium,2 strains of Aspergillus,and the remaining 7 strains of Penicillium.The phylogenetic tree showed that strain P-9 belonged to one group,the other nine strains to another group and they have different levels of the phylogenetic relationships.The main patulin-producing strains are Penicillium,and the P-1 has the strongest ability.