油菜蜂花粉蛋白的分离提取及其抗氧化活性

以油菜蜂花粉为研究对象,根据溶解性的不同,从中分离出了水溶性蛋白、醇溶性蛋白、碱溶性蛋白及盐溶性蛋白,并对各蛋白组分抗氧化活性进行了研究。结果表明:油菜蜂花粉中主要蛋白为水溶性蛋白,占其干重的7.35%,其次为醇溶性蛋白3.65%,盐溶性蛋白和碱溶性蛋白分别占2.50%和2.24%。4种不同溶解性的油菜蜂花粉蛋白均具有一定的体外抗氧化作用,各蛋白组分的总抗氧化能力以及对DPPH自由基、OH.和O2-.的清除能力呈现较好的量效关系;4种蛋白抗氧化活性由强到弱的顺序依次为水溶性蛋白>醇溶性蛋白>碱溶性蛋白>盐溶性蛋白。因此,油菜蜂花粉蛋白质可作为一种潜在的天然抗氧剂进行深入研究。

Study on Extraction and Antioxidant Activity Analysis of Rape Bee Pollen Protein

In this paper,rape bee pollen was used as the research object.Four types of rape bee pollen protein,including water-soluble,ethanol-soluble,salt-soluble and alkali-soluble protein were extracted by their different dissolving abilities.The antioxidant activities were investigated.The results showed that the contents of water-soluble protein,ethanol-soluble protein,alkali-soluble protein and salt-soluble protein were 7.35 %,3.65 %,2.50 %,and 2.24 %,respectively.All four protein components from rape bee pollen had antioxidant activity.The relationship between concentration and the total antioxidant power,the scavenging power on DPPH,OHo,and O-2o all showed positive correlation with the concentration.Antioxidant activity from strong to weak was in the following order: water-soluble protein>ethanol-soluble protein>alkali-soluble protein>salt-soluble protein.Therefore,rape bee pollen proteins have the potential as natural antioxidant.