大孔树脂纯化喀什小檗花色苷 |
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引用本文: | 苏亚丽,杨玲.大孔树脂纯化喀什小檗花色苷[J].食品与发酵工业,2011,37(11). |
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作者姓名: | 苏亚丽 杨玲 |
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作者单位: | 塔里木大学,新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔843300 |
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基金项目: | 新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放课题 |
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摘 要: | 研究XAD-7HP大孔树脂分离纯化喀什小檗花色苷的纯化工艺条件。通过树脂筛选和单因素实验确定适宜工艺参数:吸附时间是4h,体积分数60%乙醇溶液为洗脱液,解析时间100min,上样液pH值2.0,吸附温度为30℃,上样质量浓度为750mg/L,洗脱流速为1.0mL/min,。该工艺生产的花色苷产品为紫黑色粉末,色价和花色苷含量比纯化前分别提高了2.21倍和3.15倍。
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关 键 词: | 喀什小檗 花色苷 大孔树脂 纯化 |
Purification of Anthocyanins from Berberis kaschgarica by Macroporous Resins |
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Abstract: | The condition of purification of anthocyanins from Berberis kaschgarica by XAD-7HP macroporous resins was studied.The tests of screened resins and single-factor were used to determin the optimal technological parameters: 4 hours of adsorption time,60%ethanol as eluant,desorption time 100 min,pH 2.0,temperature 30℃.The concentration of anthocyanins is 750 mg /L and flow rate is 1.0 mL/min.Under the above optimum conditions,content of anthocyanins and colour value increased 3.15 and 2.21 times respectively in dark purple powder final product. |
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Keywords: | Berberis kaschgarica anthocyanins macroporous resins purification |
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