| 基于荧光生物传感技术检测牛奶中金黄色葡萄球菌肠毒素B |
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| 引用本文： | 王美玲,胡 婷,李昌哲,周焕英,高志贤,罗 鹏.基于荧光生物传感技术检测牛奶中金黄色葡萄球菌肠毒素B[J].食品安全质量检测技术,2024,15(1):57-64. |
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| 作者姓名： | 王美玲 胡 婷 李昌哲 周焕英 高志贤 罗 鹏 |
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| 作者单位： | 贵州医科大学公共卫生与健康学院,贵州医科大学公共卫生与健康学院,贵州医科大学公共卫生与健康学院,军事科学院军事医学研究院环境医学与作业医学研究所,军事科学院军事医学研究院环境医学与作业医学研究所,贵州医科大学公共卫生与健康学院 |
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| 基金项目： | 国家重点研发计划项目(2022YFF1101103)、贵州省科技厅基金项目(黔科合基础-ZK[2023]一般 318)、贵州省教育厅青年科技人才成长项目(黔教合 KY 字[2022]218) |
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| 摘 要： | 目的 利用纳米金粒子(gold nanoparticles,AuNPs)荧光猝灭性能和核酸适配体的高亲和力,构建一种简便、灵敏的纳米金“Turn-on”型荧光生物传感方法检测牛奶中金黄色葡萄球菌肠毒素B(staphylococcal enterotoxins B,SEB)的方法。方法 以AuNPs作为荧光体的能量受体(猝灭剂),荧光素-单链DNA(fluorescein-ssDNA,FAM-ssDNA)作为荧光能量供体,冷冻法制备AuNPs-SEB适配体复合物,基于AuNPsSEB适配体复合物/SEB/FAM-ssDNA的竞争性结合,构建纳米金“Turn-on”型荧光生物传感检测方法。对缓冲体系pH和反应时间等条件进行优化,以牛奶为代表对方法检测性能进行验证。结果 在优化好的实验条件(pH 7.5、反应时间15 min和反应温度25℃)下,在10-1～104 ng/mL范围内,荧光强度与SEB质量浓度之间呈现良好的线性关系,其相关系数为0.995,检出限为0.062 ng/mL。应用于牛奶样品中SEB的测定,方法回收率为91.2%～108.0%,相对标准偏差在2.6%～5.2%范围之间...
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| 关 键 词： | 纳米金粒子 核酸适配体 荧光生物传感 金黄色葡萄球菌肠毒素B 牛奶 |
| 收稿时间： | 2023/10/17 0:00:00 |
| 修稿时间： | 2023/12/14 0:00:00 |
Detection of staphylococcal enterotoxins B in milk based on fluorescence biosensing technique |
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| WANG Mei-Ling,HU Ting,LI Chang-Zhe,ZHOU Huan-Ying,GAO Zhi-Xian,LUO Peng.Detection of staphylococcal enterotoxins B in milk based on fluorescence biosensing technique[J].Food Safety and Quality Detection Technology,2024,15(1):57-64. |
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| Authors: | WANG Mei-Ling HU Ting LI Chang-Zhe ZHOU Huan-Ying GAO Zhi-Xian LUO Peng |
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| Affiliation: | School of Public Health,the Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,School of Public Health,the Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,School of Public Health,the Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety,Tianjin Institute of Environmental and Operational Medicine,School of Public Health,the Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,School of Public Health,the Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University |
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| Abstract: | |
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| Keywords: | gold nanoparticles nucleic acid aptamer fluorescent biosensing staphylococcal enterotoxins B milk |
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