酶法生产稀有人参皂甙及其产物成分的分析

通过实验研究从人参提取人参皂甙，用硅胶柱法分离人参二醇类皂甙。酶法改变人参二醇皂甙糖基，生产稀有人参皂甙，并分离提纯了其酶反应产物。结果表明：酶反应产物中主要成分为人参皂甙Rh2，副产物为人参皂甙Rg3、Rg5、Rh1、Rh3、人参二醇类皂甙元；经硅胶柱分离酶解产物得到单体。也探讨了酶法产生各种稀有人参皂甙的机理，二醇类皂甙失去第20碳上的糖基时，也会失去水分子：Rh2生成的同时Rh2失去水分子变成Rh3皂甙；Rh3生成的同时，Rg3失去水分子变成Rg5皂甙。

Preparing rare ginsenoside from enzyme reaction and composition analysis of its products

We study the processes of isolating ginsenosides from ginseng and separating protopanaxdiol type ginsenosides from ginsenoside by silica gel column method.Rare ginsenosides are obtained by enzyme hydrolysis methods from protopanaxtiol saponins. The main product from enzyme reaction is ginsenoside Rh2 and the byproducts are ginsenoside Rg3, Rg5, Rh1, Rh3 and aglycone. These rare ginsenosides are also separated with the silica gel column to single ginsenoside. It is shown from enzyme reaction, when the sugar moiety is at 20-C side of ginsenoside such as Rb, Rc, and Rd are hydrolyzed, the hydroxyl is easily lost to ginsenoside Rh3 and the ginsenoside Rg3, to Rg5.