基于HPLC指纹图谱及聚类分析对不同产地枸杞质量评价研究

基于指纹图谱与系统聚类分析方法对不同产地枸杞进行质量评价。利用高效液相色谱法（HPLC）建立8个不同产地枸杞的指纹图谱，使用相似度评价软件及SPSS19．0软件进行数据处理，对不同产地的枸杞样品进行分析。结果表明，不同产地构杞的黄酮类物质指纹图谱具有专属性，其中中宁、青海、新疆3个产地的枸杞指纹图谱相似度较高，分别为1、0．963、0．956，聚类分析也将该三个产地的枸杞聚在—起。青海、新疆产枸杞与中宁枸杞质量相似，可以作为枸杞药材新来源。HPLC指纹图谱结合聚类分析法能够对枸杞的产地识别及质量评价提供参考。

Study on Quality Evaluation of Lycium chinense from Different Areas by HPLC Fingerprint and Cluster Analysis

HPLC fingerprint ofLycium chinense and the cluster analysis was used to evaluate Lycium chinense from different areas. The chromatographic fingerprints of Lycium chinense from 8 different origins were established by HPLC. ＂Similarity Evaluation System for Chromatographic Fingerprint of TCM＂ and SPSS 19.0 was applied to analyzing Lycium chinense samples from different regions. Results showed that fmgerprints of flavonoids in Lycium chinense from different areas were specific, and the high similarities of the samples from Zhongning, Qinghai and Xinjiang were 1, 0.963 and 0.956, respectively, and they also got together in cluster analysis. Lycium chinense produced in Qinghai and Xinjiang were so similar to that in Zhongning, which could be the new source for medicine use. The method of HPLC fingerprint bonded with cluster analysis was able to provide a reference for the discrimination and quality evaluation ofLycium chinense.