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Aflatoxin and Cyclopiazonic Acid Production by Aspergillus flavus Isolated from Contaminated Maize
Authors:Y JENNIFER LEE  WINSTON M HAGLER  JR
Affiliation:Authors Lee and Hagler are with the Mycotoxin Laboratory, Dept. of Poultry Science, North Carolina State Univ., 1320 Varsity Drive, Raleigh, NC 27695-7636. Address inquiries to Dr. Hagler.
Abstract:Seven truck-loads of maize were tested for mycotoxin contamination. Aflatoxin was identified in all 7 at concentrations from 3 ng/g-501 ng/g (aflatoxin B1+ B2). Cyclopiazonic acid was identified in 4 loads with concentrations from 25-250 ng/g. Deoxynivalenol was found in 4 of 5 loads tested, over a range of 46-676 ng/g. Ninteeen isolates of Aspergillus flavus from the samples were tested for ability to accumulate cyclopiazonic acid and aflatoxin in liquid culture. Fourteen produced cyclopiazonic acid (0.5-135 μg/mL), 12 produced aflatoxin (0.01-0.70 μg/mL, aflatoxin B1+ B2), and one aflatoxin-producing isolate did not produce cyclopiazonic acid.
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