Functional Characterization of Ubiquitination Genes in the Interaction of Soybean—Heterodera glycines |
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| Authors: | Nawei Qi Jichen Yan Piao Lei Xiaofeng Zhu Xiaoyu Liu Yuanhu Xuan Haiyan Fan Yuanyuan Wang Lijie Chen Yuxi Duan |
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| Affiliation: | 1.Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, China;2.College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China;3.Institute of Plant Protection, Liaoning Academy of Agriculture Sciences, Shenyang 100161, China;4.College of Sciences, Shenyang Agricultural University, Shenyang 110866, China;5.College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China |
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| Abstract: | Ubiquitination is a kind of post-translational modification of proteins that plays an important role in plant response to biotic and abiotic stress. The response of soybean GmPUB genes to soybean cyst nematode (SCN, Heterodera glycines) infection is largely unknown. In this study, quantitative real-time PCR (qRT-PCR) was performed to detect the relative expression of 49 GmPUB genes in susceptible cultivar William 82 and resistant cultivar Huipizhi after SCN inoculation. The results show that GmPUB genes responded to cyst nematode infection at 1 day post-inoculation (dpi), 5 dpi, 10 dpi and 15 dpi. The expression levels of GmPUB16A, GmPUB20A, GmCHIPA, GmPUB33A, GmPUB23A and GmPUB24A were dramatically changed during SCN infection. Furthermore, functional analysis of these GmPUB genes by overexpression and RNAi showed that GmPUB20A, GmPUB33A and GmPUB24A negatively regulated soybean resistance under SCN stress. The results from our present study provide insights into the complicated molecular mechanism of the interaction between soybean and SCN. |
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| Keywords: | ubiquitination GmPUBs soybean Heterodera glycines soybean cyst nematode |
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