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Lysophosphatidylinositol Induced Morphological Changes and Stress Fiber Formation through the GPR55-RhoA-ROCK Pathway |
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| Authors: | Keisuke Nakajima Saori Oka Takashi Tanikawa Yoko Nemoto-Sasaki Naoki Matsumoto Hiroki Ishiguro Yoichiro Arata Takayuki Sugiura Atsushi Yamashita |
| Affiliation: | 1.Faculty of Pharma-Science, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan;2.Pharmaceutical Department, Teikyo University Hospital, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8606, Japan;3.Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado 350-0295, Japan;4.Department of Clinical Genetics, Graduate School of Medicine, University of Yamanashi, 1110, Shimokato, Chuo-shi 409-3821, Japan |
| Abstract: | We previously reported that lysophosphatidylinositol (LPI) functions as an endogenous agonist of GPR55, a novel cannabinoid receptor. However, the physiological roles of LPI-GPR55 have not yet been elucidated in detail. In the present study, we found that LPI induced morphological changes in GPR55-expressing HEK293 cells. LPI induced the cell rounding of GPR55-expressing HEK293 cells but not of empty-vector-transfected cells. LPI also induced the activation of small GTP-binding protein RhoA and increased stress fiber formation in GPR55-expressing HEK293 cells. The inhibition of RhoA and Rho kinase ROCK by the C3 exoenzyme and the ROCK inhibitor reduced LPI-induced cell rounding and stress fiber formation. These results clearly indicated that the LPI-induced morphological changes and the assembly of the cytoskeletons were mediated through the GPR55-RhoA-ROCK pathway. |
| Keywords: | lysophosphatidylinositol GPR55 endocannabinoid lysophospholipid mediator morphological change G12/13-RhoA-ROCK pathway |