万古霉素和去甲万古霉素检测方法研究进展

万古霉素和去甲万古霉素化学结构、药理性质和抗菌作用相似,均属于糖肽类抗生素,被广泛应用于细菌感染的治疗,尤其是一些超级细菌。临床上,药物治疗方案是依据血液中药物含量而确立的。另外药物的不合理使用会造成其在农产品中的残留,对人们的食用安全具有危害性,因此需要对农产品中药物残留进行监测,并建立高效的检测方法刻不容缓。本文对万古霉素和去甲万古霉素的检测方法进行综述,分别介绍了万古霉素在医疗卫生领域和动物源食品中不同的检测方法,包括高效液相色谱法(high performance liquid chromatography,HPLC)、液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)、酶放大免疫法(enzyme-multiplied immunoassay technique,EMIT)、酶联免疫法(enzyme linked immunosorbent assay,ELISA)、荧光偏振免疫法(fluorescence polarization immunoassay,FPIA)等,详细介绍了上述方法的检测原理、研究现现状及实际应用情况,并对其发展趋势进行了展望。

Research progress on analytical methods of vancomycin and norvancomycin

Vancomycin and norvancomycin, glycopeptide antibiotics with similar chemical structure, pharmacological properties and antibacterial effects are used active against infections by bacteria, particularly some super bacteria. Therapeutic drug monitoring of vancomycin and norvancomycin in plasma is highly recommended for optimizing therapy. Due to unreasonable misusing drugs result in drugs residues in agricultural products, which is harmful to human health, and therefore monitoring of drug residues in agricultural products, and establishing effective detection methods are essential. In this paper, over the past decades many methods of vancomycin and norvancomycin has been development and validated in the field of health and animal-derived food. These include high performance liquid chromatography (HPLC), liquid chromatography-tandem mass spectrometry (LC-MS/MS), enzyme immunoassay (EMIT), enzyme linked immunosorbent assay (ELISA), and fluorescence polarization immunoassay (FPIA), etc. The detection principles, research development and practical applications of these detection methods were detailly introduced, and the development trendency were prospected.