一种微生物絮凝剂急性毒性和诱变毒性作用的研究

目的研究一种微生物絮凝剂受试物的急性毒性和诱变毒性作用。方法分别对微生物絮凝剂进行了小鼠急性经口毒性试验、Ames试验、小鼠骨髓嗜多染红细胞微核试验。结果该微生物絮凝剂的小鼠急性经口毒性试验LD_(50)≥5000 mg/(kg BW);Ames试验中,各剂量组回复突变菌落数均未见明显增加,亦无剂量-反应关系;小鼠骨髓嗜多染红细胞微核试验中,微生物絮凝剂各剂量组微核细胞率与阴性对照组比较差异无显著性。结论该微生物絮凝剂对昆明种小鼠的急性经口毒性属实际无毒,在Ames试验中未呈现致突变作用,未见诱导小鼠骨髓嗜多染红细胞微核形成的能力。

Acute toxicity and mutagenicity of a microbial flocculant

Objective To investigate the acute toxicity and potential mutagenicity of a microbial flocculant. Methods The mouse acute oral toxicity test, Ames test and bone marrow polychromatic erythrocyte micronucleus test were carried out on microbial flocculants. Results In mouse acute oral toxicity test, LD50 was higher than 5000 mg/(kg BW). Compared to the negative control group, the treatment group showed an absence of mutagenicity in the Ames test, and no dose-response relationship. No significant difference among testing groups and negative control group was found in mouse bone marrow poly chromatic erythrocyte micronucleus test. Conclusion The microbial flocculant shows non-toxic effects in mouse acute test, has no sign to indicate a reverse mutation in Ames test, and has no potential capacity to induce micronucleus formation in mouse bone marrow polychromatic erythrocyte.