活性乳酸菌制品中乳酸菌计数培养条件探讨

为快速、准确地计数活性乳酸菌制品中的乳酸菌，用CO2烛缸法代替现行国家标准GB／T16347－1996活性乳酸菌饮料中乳酸菌微生物检验方法中的有氧培养法，CO2烛缸法操作简便，计算准确可靠，在菌种对比实验中，CO2烛缸法的乳酸菌计数结果是国家标准方法的8倍，在试样的对比实验中，CO2烛缸法的乳酸菌计数结果是国家标准方法的20倍，CO2烛缸法较目前国家标准方法更能真实施反映活性乳酸菌制品中乳酸菌的数量。

A method of lactic acid bacteria counting in living lactic acid bacteria products

In order to count lactic acid bacteria in living lactic acid bacteria products accurately, we substituted the aerobic culture method adopted in present national standard in China with CO 2 method ( removing oxygen from an airtight container by burning of candle). In bacterial strain comparison experiments, the counting result of lactic acid bacteria by CO 2 method was 8 times more than that by aerobic culture method. In sample comparison experiments, the counting result of lactic acid bacteria by CO 2 method was 20 times more than that by aerobic culture method. The result suggested that the CO 2 method better than aerobic culture method with its simplicity, rapidity and accuracy.