小球藻Chlorella sp.的小分子有机酸/醇培养研究

该研究选择一株对水解酸化液耐受性强、高产油脂的优良藻株Chlorella sp.作为出发藻株，向BG11培养基中分别添加不同质量浓度小分子有机酸/醇（乙酸、丙酸、丁酸、异丁酸、戊酸、异戊酸和乙醇）进行小球藻纯培养试验。在单因素试验的基础上，混合乙酸、丙酸、丁酸和乙醇4种小分子有机物质进行响应面的试验，考察混合小分子有机酸对小球藻Chlorella sp.生长影响，研究表明，当乙酸、丙酸、丁酸和乙醇的质量浓度分别为1.02 g/L、0.42 g/L、0.40 g/L和0.18 g/L时，小球藻的单位体积细胞数含量最高，生长状态最好，能获得较高的生物量（1.18×108 CFU/mL）。

Study on the cultivation of Chlorella sp. using small molecular organic acid/alcohol

Chlorella sp. pure culture experiments was carried out by using the strain with tolerance properties on hydrolysis-acidification liquid and high lipid yield and adding different concentration of small molecule organic acid/alcohol (acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and ethanol) into BG11 medium. On the basis of single factor experiments, the effect of mixed small molecule organic acids/alcohol (acetic acid, propionic acid, butyric acid and ethanol) on growth of Chlorella sp. was investigated by response surface experiments. The research indicated that when the concentration of acetic acid, propionic acid, butyric acid and ethanol were 1.02 g/L, 0.42 g/L, 0.40 g/L and 0.18 g/L, respectively, Chlorella sp. had a highest cell number, optimum growth situation and higher biomass (1.18×108 CFU/ml).