Electron microscopic investigation on the osteogenesis at titanium implant/bone marrow interface under masticatory loading |
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Authors: | H Kawahara S Nakakita M Ito K Niwa D Kawahara S Matsuda |
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Affiliation: | (1) Institute of Clinical Materials, 1-22-27 Tokocho, Moriguchi, Osaka 570-0035, Japan;(2) Ehime University School of Medicine, Sitsukawa, Shigenobu, Ehime 791-0295, Japan |
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Abstract: | Electron microscopic investigation on osteogenetic process at the implant surface of threadless rod-type titanium implants
with different surface roughness of Ra 0.4 ± 0.01 μm, Sm 2.6 ± 0.3 μm and Ra 2.0 ± 0.12 μm, Sm 36 ± 9.1 μm was performed at
the early stage of 21 and 42 days post implantation into the jawbones of four beagles under the load bearing condition of
functional mastication. The implant surfaces were covered with a blood clot and haematopoietic stem cells (HSC) including
phagocytic monocytes immediately after the implantation. Successively, osteogenic stem cells (OSC) migrated from cortical
and/or trabecular endosteum to the HSC-layer on the implant surface. The new bone formation at the implant/bone marrow interface
was developed by collaboration of osteomediator cells (OMC) differentiated from monocytes of HSC and osteoblast phenotype
cells of OSC derived from endosteum of cortical bone and/or trabecular. The new bone layer at the implant surface consisted
of two layers, solution-mediated calcification layer of pseudo bone and cell (osteoblast) -mediated calcification layer of
true bone. The pseudo bone was produced by solution-mediated calcification of OMC- and HSC-remnants near by the implant surface.
The bone healing process at the implant/bone marrow interface depended upon two factors; the migration of OSC from cortical
and/or trabecular endosteum to the implant surface and the healing potentiality. Topographic dependency upon the bone healing
potential at implant/bone marrow interface was not confirmed in this experiment under the load bearing condition of functional
mastication. |
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