Lipid Profiling Reveals Tissue-Specific Differences for Ethanolamide Lipids in Mice Lacking Fatty Acid Amide Hydrolase |
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Authors: | Aruna Kilaru Giorgis Isaac Pamela Tamura David Baxter Scott R Duncan Barney J Venables Ruth Welti Peter Koulen Kent D Chapman |
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Affiliation: | (1) Department of Biological Sciences, Center for Plant Lipid Research, University of North Texas, 1155 Union Circle #305220, Denton, TX 76203-5017, USA;(2) Division of Biology, Kansas Lipidomics Research Center, Kansas State University, Ackert Hall, Manhattan, KS 66506-4901, USA;(3) Departments of Basic Medical Science and Ophthalmology, School of Medicine, University of Missouri-Kansas City, 2411 Holmes Street, Kansas City, MO 64108, USA;(4) Present address: Department of Plant Biology, Michigan State University, 366 Plant Biology Building, East Lansing, MI 48824, USA;(5) Present address: Pacific Northwest National Laboratory, PO Box 999, MSIN: K8-98, Richland, WA 99352, USA; |
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Abstract: | N-Acylethanolamines (NAE) are fatty acid derivatives, some of which function as endocannabinoids in mammals. NAE metabolism
involves common (phosphatidylethanolamines, PEs) and uncommon (N-acylphosphatidylethanolamines, NAPEs) membrane phospholipids. Here we have identified and quantified more than a hundred
metabolites in the NAE/endocannabinoid pathway in mouse brain and heart tissues, including many previously unreported molecular
species of NAPE. We found that brain tissue of mice lacking fatty acid amide hydrolase (FAAH
−/−) had elevated PE and NAPE molecular species in addition to elevated NAEs, suggesting that FAAH activity participates in the
overall regulation of this pathway. This perturbation of the NAE pathway in brain was not observed in heart tissue of FAAH
−/− mice, indicating that metabolic regulation of the NAE pathway differs in these two organs and the metabolic enzymes that
catabolize NAEs are most likely differentially distributed and/or regulated. Targeted lipidomics analysis, like that presented
here, will continue to provide important insights into cellular lipid signaling networks. |
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