| 中国野桑蚕mtDNA中Cyt b基因的克隆及序列分析 |
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| 引用本文： | 刘波,郑小坚,薛仁宇,曹广力,贡成良.中国野桑蚕mtDNA中Cyt b基因的克隆及序列分析[J].苏州大学学报(工科版),2007,27(1):1-6. |
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| 作者姓名： | 刘波 郑小坚 薛仁宇 曹广力 贡成良 |
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| 作者单位： | 苏州大学生命科学学院,江苏,苏州,215123 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 通过PCR的方法,对中国青州野桑蚕mtDNA中Cytb基因及侧翼序列进行克隆和序列分析。所获得的序列长度为1761nt,含有完整的Cytb基因t、RNA-ser基因及ND6和ND1基因的部分序列。同源性分析显示,所测定的Cytb基因与中国安康野桑蚕、日本野桑蚕、中系夏芳家蚕、中系C-108家蚕、日系Aojuku家蚕和韩国Backokjam家蚕的Cytb基因具有很高的同源性;基于线粒体Cytb基因的核苷酸序列的分子系统进化分析显示,中国青州野桑蚕与家蚕的亲缘关系较近,表明家蚕可能起源于中国野桑蚕。
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| 关 键 词： | 野桑蚕 细胞色素b基因 分子进化 |
| 文章编号： | 1673-047X（2007）01-0001-06 |
| 收稿时间： | 2006-07-10 |
| 修稿时间： | 2006年7月10日 |
Cloning and Sequence Analysis of the Cyt b Gene of mtDNA from Chinese Bombyx mandarina M. |
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| LIU Bo,ZHENG Xiao-Jian,XUE Ren-Yu,CAO Guang-Li,GONG Cheng-Liang.Cloning and Sequence Analysis of the Cyt b Gene of mtDNA from Chinese Bombyx mandarina M.[J].Journal of Suzhou University(Engineering Science Edition),2007,27(1):1-6. |
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| Authors: | LIU Bo ZHENG Xiao-Jian XUE Ren-Yu CAO Guang-Li GONG Cheng-Liang |
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| Affiliation: | College of Life Science, Soochow University, Suzhou 215123, China |
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| Abstract: | The Cyt b gene and its flank region(1761nt) of mitochondrial DNA(mtDNA) from the Chinese Qingzhou Bombyx mandarina were cloned using PCR.Sequence result revealed the fragment contained an intact Cyt b gene,tRNAser(UCN) gene,and partial sequence of ND6 and ND1 gene.Homologous comparison of Cyt b genes from Chinese Ankong Bombyx mandarina with Japanese Bombyx.mandarina,Chinese Bombyx mori(strain Xiafang),Chinese Bombyx mori(strain C108),Japanese Bombyx mori(strain Aojuku) and Korean Bombyx mori respectively showed high homology.Molecular phylogenetic analysis based on the sequence of Cyt b gene reflected the relationship between Qingzhou Bombyx mandarina and Bombyx mori was closer,it indicated that Bombyx mori possibly derivated from Chinese Bombyx mandarina. |
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| Keywords: | mtDNA |
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