蛋白质分配系数的实验测定和热力学模型计算

本文实验测定了６种蛋白质在电解质／高聚物双水相体系中的４组分配系数。在Ｅａｓｋｉｒ的蛋白质球形晶格模型基础上计算了蛋白质和水、高聚物链段的短程作用，用Ｐｉｔｚｅｒ－Ｌｉ长程静电项计算它们的长程作用。由１组分配系数回归多数预测另外３组分配系数，其预测值和实验值的平均相对误差低于６％。并讨论了蛋白质在双水相体系中分配系数的影响因素。

EXPERIMENTAL MEASUREMENT AND THERMO-DYNAMIC MODEL CALCULATION OF PROTEIN PARTITION COEFFICIENT

he four sets of protein partition coefficients of six kil1ds of pro-tein in electrolyte/polymer two-phase aqueous system were experimentally mea-sured at 25℃. On the basis of Baskir protein global lattice model, the short-range interaction between protein and water or polymer chain is calculated.The Pitzer-Li long range electrostatic term is used to calculate the long range interaction,Three sets of partition coefficients are predicted by regre-ssion parameters of one set of partition coefficients. The average relative de-viation between prediction and experiment is less than 6%,The infll1encc factors of protein partition coefficient in two-phase aqueous system are discussed.