双功能菌群在金属矿区黄药、镉复合污染土壤修复中的应用

金属矿区典型浮选药剂黄药和重金属Cd具有易蓄积、高迁移等特性,导致矿区周边土壤产生严重复合污染,但目前仍缺乏对于其进行高效、绿色修复的技术。对双功能菌群SDMC修复丁基黄药和Cd复合污染土壤的效果及其对土壤微生物特征的影响进行了研究,结果表明,菌群SDMC对污染土壤中丁基黄药降解率、Cd矿化率分别达到63.34%、71.83%,具有良好的修复效果。同时发现菌群修复对土壤微生物丰度、酶以及代谢活性均表现出显著的促进作用,与未修复土壤相比,土壤微生物丰度、过氧化氢酶、脲酶、蔗糖酶活性以及AWCD分别提高了9、1.22、5.92、1.15和1.18倍;具有降解矿化复合污染物作用的芽孢杆菌属、生丝微菌属和假单胞菌属的相对丰度分别提高了43.75个百分点、21.32个百分点和4.25个百分点,表明该菌群已成为修复优势菌。研究结果为金属矿区黄药和Cd复合污染土壤修复提供了一种新的方法。

Application of Bifunctional Bacterial Flora in the Remediation of Butyl Xanthate and Cd Compound Contamination Soil in Metal Mining Areas

Typical flotation chemicals butyl xanthate and heavy metal Cd in metal mining areas have the characteristics of easy accumulation and high migration,which lead to serious compound pollution of the soil around the mining area,but there is still a lack of efficient and green remediation technology for them.The effectiveness and soil microbial characteristics of the bifunctional flora SDMC in remediating the compound contamination soil with butyl xanthate and Cd were studied,and the results showed that the degradation rate of butyl xanthate and Cd mineralization rate could reach 63.34% and 71.83%,respectively,with good remediation effect.The soil microbial abundance,peroxidase,urease,sucrase activity,and AWCD increased by 9,1.22,5.92,1.15 and 1.18 times,respectively,compared with the non-remediated soil,indicating significant facilitation for soil microbial abundance,enzymatic and metabolic activity.The relative abundance of Sporosarcina,Hyphomicrobium and Pseudomonas,which play a role in degrading mineralized composite pollutants,increased by 43.75 percentage points,21.32 percentage points and 4.25 percentage points,respectively,indicating that SDMC has become the dominant bacteria for remediation,and this study provides a new method for remediation of butyl xanthate and Cd compound pollution soil in metal mining areas.