Fast analysis of rapeseed glucosinolates by near infrared reflectance spectroscopy |
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Authors: | R Biston P Dardenne M Cwikowski M Marlier M Severin J-P Wathelet |
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Affiliation: | 1. Station de Haute Belgique, rue de Serpont, 48, B-6600, Libramont, Belgium 2. Faculté des Sciences agronomiques de l’Etat, U.E.R. de Chimie générale et organique, B-5800, Gembloux, Belgium
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Abstract: | Industry and plant breeders require fast methods to analyze glucosinolates in rapeseed. We tested the potential of near infrared
reflectance spectroscopy (NIRS) for this analysis and developed calibration equations on a large population of whole seeds.
Reference methods used are high performance liquid chromatography (HPLC) and gas liquid chromatography (GLC) of desulphoglucosinolates,
a glucose-release method after purification on an anion exchange column and a palladium test. In the range from 2 to 107 μM/g
and after transformation of the data in first derivative, a correlation coefficient of 0.99 was observed, as well as standard
errors of estimated values of 2.15, 2.52, 2.67 and 4.07 for samples analyzed by HPLC, GLC, glucose and the palladium test,
respectively. With different wavelengths, a limited calibration test on seeds containing from 4 to 40 μM/g gives a standard
error of 1.91 μM/g (HPLC). |
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