Absence of sterol biosynthesis in oyster tissue culture |
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Authors: | Marcia J. Holden Glenn W. Patterson |
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Affiliation: | (1) Department of Botany, University of Maryland, 20742 College Park, Maryland;(2) Present address: Foreign Disease-Weed Science, Ft. detrick, USDA Agricultural Research Service Frederick, MD, 21701 |
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Abstract: | An oyster heart tissue culture was used to investigate the ability of the American oyster (Crassostrea virginica) to synthesize sterols from [14C]acetate. Lipids were extracted from cultured oyster heart cells and no label was found in the purified fatty acid fraction, 90% of which was located in the saturated and monounsaturated fractions. Although these results cannot rule out the possibility of sterol synthesis in other oyster tissues, this and previous evidence are in support of the hypothesis that the American oyster is unable to synthesize sterols. |
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