Modulation of fatty acid incorporation and desaturation by trifluoperazine in fungi |
| |
Authors: | Yasushi Kamisaka Toshihiro Yokochi Toro Nakahara Osamu Suzuki |
| |
Affiliation: | (1) Biological Chemistry Division, National Chemical Laboratory for Industry, Higashi 1-1, 305 Tsukuba, Ibaraki, Japan |
| |
Abstract: | The effects of trifluoperazine (TFP) on 1-14C]fatty acid incorporation into the lipids ofMortierella ramanniana var.angulispora were studied. TFP decreased 1-14C]-fatty acid incorporation into phosphatidylcholine, phosphatidylethanolamine and triacylglycerol, but greatly increased14C-labeling in phosphatidic acid. These changes in 1-14C]fatty acid incorporation induced by TFP were accompanied by a decrease in desaturation of some 1-14C]fatty acids taken up by the fungal cells. When 1-14C]lioleic acid (LA) was incubated with the fungal cells, total γ-linolenic acid (GLA) formation from incorporated 1-14C]LA decreased, but the14C-labeled GLA conent in individual lipid classes was essentially unchanged. This suggests that the site of the TFP effect
on GLA formation from 1-14C]LA taken up from the medium is not the desaturase acting on LA linked to complex lipids. On the other hand, GLA formation
from 1-14C]oleic acid was much less susceptile to TFP, which suggests that in this fungus Δ6 desaturation to GLA has at least two different
pathways with different degrees of susceptibility to TFP. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|