The effect of highly purified eicosapentaenoic and docosahexaenoic acids on monocyte phagocytosis in man |
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Authors: | Seeger Dag Halvorsen John-Bjarne Hansen Sameline Grimsgaard Kaare H Bønaa Peter Kierulf Arne Nordøy |
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Affiliation: | 1. Department of Medicine, Institute of Clinical Medicine, University of Troms?, N-9037, Troms?, Norway 2. Institute of Community Medicine, University of Troms?, N-9037, Troms?, Norway 3. Research and Development Group, Clinical Chemistry Department, Ollev?l University Hospital, Oslo, Norway
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Abstract: | The n-3 fatty acids (FA) from marine sources are known to exert antiinflammatory effects on monocyte function. There is still
controversy whether n-3 FA may increase the susceptibility to infections. The present study was designed to assess the effect
of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHa) on monocyte phagocytosis and respiratory burst activity. Fifty-eight
healthy men were randomized to take a daily supplement of 3.8 g highly purified EPA (n=20), 3.6 g DHA (n=19), or corn oil (n=19) for 7 wk. Mononuclear leukocytes were collected, isolated, and cryopreserved prior to and after dietary supplementation.
Paired samples were analyzed in the presence of autologous serum in a crossover design. Monocyte phagocytosis and respiratory
burst activity were measured by flow cytometry after ingestion of Escherichia coli. Monocytes retained their phagocytic ability and respiratory burst activity after supplementation. No reduction in internalization
of bacteria was registered. Dietary n-3 FA and particularly EPA improved bacterial adherence to the monocyte surface. In the
crossover experiments, there was an adverse effect of serum enriched with n-3 FA on bacterial adherence. We conclude that
monocytes retain their phagocytic potential after supplementation with purified EPA and DHA. |
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