| 广州地区海产品中创伤弧菌病原学特征分型 |
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| 引用本文： | 张晶,张欣强,周勇,陶霞,吴新伟. 广州地区海产品中创伤弧菌病原学特征分型[J]. 中国食品卫生杂志, 2016, 28(4): 422-425 |
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| 作者姓名： | 张晶 张欣强 周勇 陶霞 吴新伟 |
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| 作者单位： | 广州市疾病预防控制中心,广东 广州 510440,广州市疾病预防控制中心,广东 广州 510440,广州市疾病预防控制中心,广东 广州 510440,广州市疾病预防控制中心,广东 广州 510440,广州市疾病预防控制中心,广东 广州 510440 |
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| 基金项目： | 广州市医学重点学科建设项目(2013-2015-07)；广州市医药卫生科技项目(20141A011054) |
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| 摘 要： | 目的研究广州地区海产品中分离的创伤弧菌的毒力相关基因与脉冲场凝胶电泳(PFGE)分子分型状况,初步建立区域PFGE溯源数据库,为该菌的分子流行病学研究和食源性疾病溯源提供支持。方法对68株创伤弧菌进行毒力相关基因鉴定和PFGE分型,采用Bio Numerics软件分析聚类关系。结果海产品中分离的创伤弧菌均为溶血素A基因(vvh A)核酸阳性,vcg C/E和16S rRNA A/B分型结果显示,共有5种基因型别,分别有CB型、EA型、CAB型、CA型和EB型。共检测出42株为BT1型。PFGE分型结果显示68株创伤弧菌有52种PFGE型别。结论广州地区海产品中分离的创伤弧菌PFGE型别多,且相互之间关系分散;毒力相关基因型以CB型为主。
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| 关 键 词： | 创伤弧菌 毒力相关基因 脉冲场凝胶电泳 分型 广州 食源性致病菌 海产品 关键词:食品 基质 亚硝酸盐 分光光度法 测定 |
| 收稿时间： | 2015-12-07 |
Pathogenic biological characteristics of Vibrio vulnificus isolates from seafood in Guangzhou |
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| ZHANG Jing,ZHANG Xin-qiang,ZHOU Yong,TAO Xia and WU Xin-wei. Pathogenic biological characteristics of Vibrio vulnificus isolates from seafood in Guangzhou[J]. Chinese Journal of Food Hygiene, 2016, 28(4): 422-425 |
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| Authors: | ZHANG Jing ZHANG Xin-qiang ZHOU Yong TAO Xia WU Xin-wei |
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| Affiliation: | Guangzhou Center for Diseases Control and Prevention,Guangdong Guangzhou 510440,China,Guangzhou Center for Diseases Control and Prevention,Guangdong Guangzhou 510440,China,Guangzhou Center for Diseases Control and Prevention,Guangdong Guangzhou 510440,China,Guangzhou Center for Diseases Control and Prevention,Guangdong Guangzhou 510440,China and Guangzhou Center for Diseases Control and Prevention,Guangdong Guangzhou 510440,China |
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| Abstract: | To analyze the virulence related-genes and pulsed-field gel electrophoresis (PFGE) characteristics of Vibrio vulnificus isolates in Guangzhou, and to establish regional PFGE database which would support molecular epidemiology of Vibrio vulnificus and traceability of foodborne disease.Methods 68 Vibrio vulnificus strains were characterized by the virulence related-genes and PFGE typing. Finger printing of each strain was analyzed by BioNumerics. Results All Vibrio vulnificus strains were positive for hemolysis A gene (vvhA).Five genetypes were detected, they were CB, EA, CAB, CA and EB type. 42 BT1 nucleic acid positive strain were detected. The 68 strains of Vibrio vulnificus were divided into 52 PFGE patterns.Conclusion The PFGE pattern of Vibrio vulnificus isolated from seafood was diversed, and CB type was the predominant virulence related-gene. It is necessary to strengthen the detection of Vibrio vulnificus in marine products to prevent the outbreak of Vibrio vulnificus infection. |
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