Fatty acids of cows' milk. B. Composition by gas-liquid chromatography aided by other methods of fractionation |
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Authors: | S F Herb Paul Magidman Francis E Luddy R W Riemenschneider |
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Affiliation: | (1) Eastern Regional Research Laboratory, Philadelphia 18, Pennsylvania |
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Abstract: | A fresh sample of cows' milk was converted directly to the methyl esters by methanolysis and the esters were fractionated
into eleven distilled fractions and an undistilled portion. The latter, which contained the bulk of the polyunsaturated and
long chain esters, was further fractionated by adsorption chromatography on a silicic acid column. Each fraction from the
distillation and adsorption chromatography was analyzed by gas-liquid chromatography on columns containing polyester stationary
phases of different polarity. Twenty-seven minor components, including some not previously reported, were present each in
less than 0.1%. The fatty acid distribution of the major components fell within the range generally reported.
Presented at the 53rd Meeting of the American Oil Chemists' Society, October 17–19, 1960, New York.
Eastern Utilization Research and Development Division, Agricultural Research Service, United States Department of Agriculture. |
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