| 果蔬中叶酸的提取分离及测定方法研究 |
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| 引用本文： | 郭秀珠,黄品湖,雷海清,项秀珠. 果蔬中叶酸的提取分离及测定方法研究[J]. 食品科学, 2006, 27(3): 183-185 |
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| 作者姓名： | 郭秀珠 黄品湖 雷海清 项秀珠 |
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| 作者单位： | 浙江省科学院亚热带作物研究所 |
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| 摘 要： | 本文研究了果蔬中叶酸的提取分离方法,对提取液、吸附剂及洗脱液设计了正交试验,结果是用热沸偏磷酸提取,活性炭吸附,3%氨-70%乙醇液洗脱,达到提取分离较完全之目的;并用荧光法测定叶酸含量,该方法回收率在95%以上,相对偏差小于2.5%,结果满意。
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| 关 键 词： | 叶酸 荧光法 提取分离 吸附剂 |
| 文章编号： | 1002-6630（2006）03-0183-03 |
| 收稿时间： | 2005-02-25 |
| 修稿时间： | 2005-02-25 |
Study on the Extraction Purifying and Measuring Method of the Folacin in Fruits and Vegetables |
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| GUO Xiu-zhu,HUANG Pin-hu,LEI Hai-qing,XIANG Xiu-zhu. Study on the Extraction Purifying and Measuring Method of the Folacin in Fruits and Vegetables[J]. Food Science, 2006, 27(3): 183-185 |
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| Authors: | GUO Xiu-zhu HUANG Pin-hu LEI Hai-qing XIANG Xiu-zhu |
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| Affiliation: | Institute of Subtropical Crops, Zhejiang Academy of Sciences, Wenzhuo 325005, China |
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| Abstract: | In this paper , the extracting and purifying technology of the folacin in fruits and vegetables was studied. We designedthe orthogonal test for the solvent, developing agent and sorbent. The results showed: extracted with hot boiling metaphosphoricacid , absorbed with active carbon, developed with the mixture solution of 3% ammonia and 70% anhudrous alcohol, the conclusiongained the ends of extraction and purifying comparative completeness. And we measured the content of folacin with thefluorescence spectrophotometer. The recovery ratio was beyond 95%, the relative deviation was below 2.5%. The results wassatisfied. |
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| Keywords: | folacin fluorescence analysis extraction purifying sorbent |
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