Quantitative determination of lipids and their constituents by the Chromarod TLC-FID system |
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| Authors: | Toshihiro Itoh Masamichi Tanaka Hiroshi Kaneko |
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| Affiliation: | (1) Division of Chemistry, School of General Studies, Kitasato University, 1-15-1 Kitasato, Sagamihara, 228 Kanagawa, Japan |
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| Abstract: | The methanolysis products of neutral sphingolipid and archaebacterial neutral glycolipid were separated on Chromarods S-II (silica gel) with a double developing system. The lipid constituents separated on the rods were scanned automatically with a hydrogen flame ionization detector (Iatroscan). The molar ratios of the constituents determined by this system were very close to the theoretical values of the lipid. |
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