Effects of preservation methods, parasites, and gut contents of black flies (Diptera: Simuliidae) on polymerase chain reaction products |
| |
Authors: | DA Koch GA Duncan TJ Parsons KP Pruess TO Powers |
| |
Affiliation: | Departamento de Tensioactivos, Centro de Investigación y Desarrollo, Consejo Superior de Investigaciones Científicas, Barcelona, Spain. |
| |
Abstract: | The vesicle to micelle transition which results in the interaction of the Triton X-100 surfactant with phosphatidylcholine vesicles was studied by means of dynamic light scattering (at different reading angles) and by freeze-fracture electron microscopy techniques. Vesicle solubilization was produced by the direct formation of mixed micelles without the formation of complex intermediate aggregates. Thus, vesicle to micelle transformation was mainly governed by the progressive formation of mixed micelles within the bilayer. A subsequent separation of these micelles from the liposome surface (vesicle perforation by the formation of surfactant-stabilized holes on the vesicle surface) led to a complete solubilization of liposomes. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|