| 大孔树脂D101固定中性脂肪酶及其生物催化应用 |
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| 引用本文： | 吴茜茜,穆文侠,孙健,吴克. 大孔树脂D101固定中性脂肪酶及其生物催化应用[J]. 食品与发酵工业, 2008, 34(8) |
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| 作者姓名： | 吴茜茜 穆文侠 孙健 吴克 |
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| 作者单位： | 合肥学院生物与环境工程系,安徽,合肥,230022 |
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| 基金项目： | 安徽省科技厅科技攻关计划项目，合肥学院应用酶学与工程重点实验室资助项目 |
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| 摘 要： | 以大孔树脂D101固定化中性脂肪酶,研究了固定化条件对酶催化活性的影响,得到了最佳固定化条件:给酶量为90IU/g,固定化温度为35℃,时间为12h,此时固定化酶的活力回收约为60%。固定化酶的半失活温度由游离酶的53℃提高到57℃,最适反应温度和最适pH分别由42℃上升至44℃和由7.5下降到7.3。对固定化中性脂肪酶在生物柴油合成中应用也进行了初步研究。
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| 关 键 词： | D101大孔树脂 固定化 中性脂肪酶 生物柴油 |
Lipase Immobilization on Macroporous Resin D101 and Application in Bio-synthesis |
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| Wu Qianqian,Mu Wenxia,Sun Jian,Wu Ke. Lipase Immobilization on Macroporous Resin D101 and Application in Bio-synthesis[J]. Food and Fermentation Industries, 2008, 34(8) |
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| Authors: | Wu Qianqian Mu Wenxia Sun Jian Wu Ke |
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| Abstract: | Neutral lipase was immobilized on macroporous resin D101 and the optimization of immobilizing conditions were investigated.The optimized results were achieved with 90IU/g of the amount of lipase to the resin in weight,coupling temperature of 35℃ and immobilizing time of 12h.The maximum yield of immobilized enzyme was 60%.In comparison with the free enzyme,the temperature to loss a half activity of the immobilized enzyme was increased from 53℃ to 57℃,the optimum temperature and optimum pH were shifted from 42℃ to 44℃ and from 7.5 to 7.3,respectively.The production of biodiesel by immobilized neutral lipase has been also investigated. |
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| Keywords: | macroporous resin D101 immobilization neutral lipase biodiesel |
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