| 加速溶剂萃取-气相色谱法快速测定食品中反式脂肪酸 |
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| 引用本文： | 徐慧,武晓静,庄件兵,贾涛,赵厚民.加速溶剂萃取-气相色谱法快速测定食品中反式脂肪酸[J].现代科学仪器,2009(2):87-89. |
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| 作者姓名： | 徐慧 武晓静 庄件兵 贾涛 赵厚民 |
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| 作者单位： | 江苏省理化测试中心,江苏省食品营养成分与有毒有害物质检测中心,南京,210042 |
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| 摘 要： | 应用加速溶剂萃取（ASE）技术对食品中的油脂进行富集，经甲酯化反应后，通过HP-88毛细管色谱柱分离测定食品中反式脂肪酸含量。结果：在提取温度125℃，提取压力1500psi，以正己烷／二氯甲影甲醇（3：2：1，V／V／V）可对食品中反式脂肪酸进行有效提取，以C16：1-t9为例，该方法加标回收率在96．1％～99．3％，相对标准偏差为0．5％～2．3％，最低检测限为2．0μg/mL，顺、反式脂肪酸分离良好。该方法简单、快速、所耗溶剂少、效率高，适用于食品中脂肪酸的测定。
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| 关 键 词： | 加速溶剂萃取 气相色谱 反式脂肪酸 |
Determination of Trans Fat Acids in Foods by ASE/GC |
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| Xu Hui,Wu Xiaojing,Zhuang Jianbing,Jia Tao,Zhao Houmin.Determination of Trans Fat Acids in Foods by ASE/GC[J].Modern Scientific Instruments,2009(2):87-89. |
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| Authors: | Xu Hui Wu Xiaojing Zhuang Jianbing Jia Tao Zhao Houmin |
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| Affiliation: | (Jiangsu Test Center for Physics and Chemistry, Jiangsu Test Center for Nutrition an Toxicant in Food Jiangsu, Nanjing 210042 ,China) |
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| Abstract: | An analytical procedure has been developed for determination of trans fat acids in foods. Fat, which was extracted using accelerated solvent extraction （ASE）, was methylated and separated on a capillary gas chromatography column of HP - 88. Hexane - dichloromethane - methanol mixed solution （3 ： 2 ： 1, V/V/V ） was used as extractant. The temperature of extraction was 125℃ and the pressure was 1500psi. The average recoveries were 96.1% -99.3% with RSD 0.5% - 2.3%. The lowest detection limit was 2.0μg/mL. The results showed that this method is simple, rapid, sensitive, and reliable, and the ASE is suitable for the determination of fat acids in foods. |
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| Keywords: | Accelerated solvent extraction Gas chromatography Trans fat acids |
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