| (R)-肾上腺素高产菌株发酵培养基的优化 |
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| 引用本文： | 陈必钦,杨凯,陈倍,阮文兵,许小平. (R)-肾上腺素高产菌株发酵培养基的优化[J]. 计算机与应用化学, 2009, 26(7) |
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| 作者姓名： | 陈必钦 杨凯 陈倍 阮文兵 许小平 |
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| 作者单位： | 福州大学化学化工学院,福建,福州,350108;福州大学化学化工学院,福建,福州,350108;福州大学化学化工学院,福建,福州,350108;福州大学化学化工学院,福建,福州,350108;福州大学化学化工学院,福建,福州,350108 |
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| 摘 要： | 为了充分发挥出突变株的高转化活性及其优良特性,采用响应面试验设计方法优化(R)-肾上腺素高产菌株Kocuria rhizophila(K.rhizophila).H5401的发酵培养基.用Plackett-Burman设计法研究初始发酵培养基中各成分对(R)-肾上腺素产率的影响,筛选出有显著效应的3个因素:甘油、蛋白胨和NH4H2PO4.利用最陡爬坡法逼近最大响应区域.通过Box-Behnken中心组合实验和响应面分析方法确定出3个主要影响因素的最佳浓度.实验结果经过回归分析拟合出1个2次方程,用其预报的最优培养基做摇瓶发酵试验,(R)-肾上腺素产率达61.07%,比优化前提高了24.89%,表明响应面试验设计优化方法有效提高了(R)-肾上腺素的产量,降低了生产成本,为后续放大实验提供依据.
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| 关 键 词： | (R)-肾上腺素 发酵培养基 Plackett-Burman 响应面分析法 |
Optimization of (R)-adrenaline high producing strain fermentation medium |
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| Chen Biqin,Yang Kai,Chen Bei,Ruan Wenbin,Xu Xiaoping. Optimization of (R)-adrenaline high producing strain fermentation medium[J]. Computers and Applied Chemistry, 2009, 26(7) |
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| Authors: | Chen Biqin Yang Kai Chen Bei Ruan Wenbin Xu Xiaoping |
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| Affiliation: | Collage of Chemistry and Chemical Engineeing;Fuzhou University;Fujian;350108;Fuzhou;China |
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| Abstract: | Aiming at increasing the rate of production,the fermentation medium of Kocuria rhizophila(K.rhizophila).H5401 highly producing(R) -adrenaline was optimized by using response surface analyzing method(RSAM).In the first step of optimization,the impact of initial medium constituents on(R)-adrenaline production was studied by Plackett-Burman design.Glycerol,Peptone and NH_4H_2PO_4 were found to be the important factors affecting(R)-adrenaline production significantly.In our work,the greatest response area was e... |
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| Keywords: | Plackett-Burman (R) -adrenaline fermentation medium Plackett-Burman response surface analyzing method |
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