大孔型纤维素硫酸钠-聚二甲基二烯丙基氯化铵微胶囊固定假丝酵母产脂肪酶

脂肪酶（1ipase，EC．3．1．1．3）能够分解或合成甘油酸酯键．近年来，随着非水相酶学研究的进展，大大扩展了脂肪酶在有机合成、外消旋体拆分等方面的用途．到目前，有关游离及固体培养产脂肪酶的方法已有较多的报道．固定化技术由于在提高产品产率和简化下游分离过程等方面特殊的优越性，也被应用于固定化发酵胞外酶．

Cultivation of encapsulated C. valida for producing lipase in macro-porous NaCS-PDMDAAC microcapsules

In order to produce macromolecules by using the immobilized cells in microcapsules, a novel macro-porous NaCS-PDMDAAC capsules system was used to immobilize Candida valida in a semicontinuous culture mode for producing lipase. It was found that the encapsulation system had no negative effect on the growth and lipase productivity, and the lipase produced inside capsules could diffuse freely to the media outside capsules. The cultivation with the capsules was carried out for 15 batches and the lipase in each batch had almost the same activity. In comparison with free cell cultivation, the immobilized cell fermentation shortened obviously the period of glucose consumption from 24 hours to 8 hours and increased lipase productivity.