响应面优化莲藕多酚提取工艺及其生物活性研究

以莲藕为原料,在单因素试验基础上,利用响应面分析优化莲藕多酚提取工艺条件,并测定其抗氧化、降血糖和降尿酸活性.结果 表明:莲藕多酚提取最优工艺参数为液料比22∶1(mL/g)、提取温度53℃、提取时间41 min,在此条件下多酚提取量为(359.55±1.20)mg/100 g.体外抗氧化活性结果显示,1,1-二苯基-...

Optimization of Extraction Process and Bioactivities of Polyphenols from Lotus Root by Response Surface Method

Based on single factor experiment,response surface methodology was used to optimize the extraction conditions of polyphenols from lotus root,and the antioxidant,hypoglycemic and uric acid reduction activities of polyphenols were determined.The results showed that the optimal extraction process parameters of lotus root polyphenols were liquid-to-material ratio 22:1(mL/g),extraction temperature 53 ℃,and extraction time 41 min.Under these conditions,the extraction amount of polyphenols was (359.55±1.20)mg/100 g.The 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) radicals and 2,2’-azinyl-bis-(3-ethylbenzothiazole-6-sulfonic acid radical (ABTS⁺))scavenging activities,ferric reducing antioxidant power (FRAP) value and N,N-dimethyl-p-phenylenediamine dihydrochloride (DMPD) radical scavenging capacity were (1292.84±9.35)μmol Trolox/g,(695.90±19.53)μmol Trolox/g,(353.31±0.99) μmol Trolox/g and (1029.15±2.54)μmol Trolox/g,respectively.The inhibition rates of α-glucosidase and α-amylase were (77.54±0.2)% and (33.86±2.0)%,respectively.The inhibition rate of xanthine oxidase was (46.87±3.1)%.In vitro experiments showed that lotus root polyphenols not only had good antioxidant activity,but also showed good effect in lowering blood glucose and uric acid.