高效液相色谱法与pH示差法测定紫荚豌豆花青素含量的比较

目的:采用高效液相色谱法(High performance liquid chromatography,HLPC)测定不同豌豆品种的花青素单体及含量,并与pH示差法测定结果进行了比较,旨在研究紫英豌豆花青素定量分析方法的差异及其花青素的种类及功能.方法:紫荚豌豆以乙醇和盐酸作为萃取剂,采用安捷伦1260高效液相色谱仪进...

Determination of Anthocyanin in Purple Pea by High Performance Liquid Chromatography and pH Differential Method

Objective:In this study,high performance liquid chromatography method was used to determine the anthocyanin monomer and content of different pea varieties,and the results were compared with those determined by pH differential method,aiming to study the difference of quantitative analysis methods of anthocyanins in purple pea,as well as the types and functions of anthocyanins.Method:Purple pea was determined by high performance liquid chromatography with ethanol and hydrochloric acid as extraction agents.Using 10% formic acid aqueous solution and 10% formic acid methanol solution as mobile phases to detect the anthocyanin in the purple pea at the wavelength of 520 nm.The pH differential method was used to determine the anthocyanin content under the conditions of 520 nm wavelength,pH buffer of 1.0 and 4.5.Results:Among the anthocyanin monomers of 6 kinds of purple peas determined by HPLC,delphinidin and cyanidin were detected in purple pea,in which delphinidin pigment was significantly higher than cyanidin.T0183 has the highest anthocyanin content in total anthocyanins detected by pH differential method.Conclusion:The results showed that the difference between HPLC and pH differential methods was related to the anthocyanins in purple pea,the selection of anthocyanins standard materials by HPLC and pH differential methods,and the low accuracy of pH differential method.In this paper,HPLC and pH differential method were used to quantitatively analyze anthocyanins in purple pea,which improves the stability and accuracy of anthocyanins detection.