Quantitative determination of 1,2-diacylglycerol in thoracic aorta of the rat using latroscan TLC/FID: Effect of norepinephrine |
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Authors: | Kenji Okumura Yumiko Yamada Junichiro Kondo Norio Kobayashi Hidekazu Hashimoto Takayuki Ito |
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Affiliation: | (1) The Second Department of Internal Medicine, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, 466 Nagoya, Japan |
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Abstract: | This study was undertaken to evaluate the use of the Iatroscan TLC/FID system for quantitating 1,2-diacylglycerol (DG) in
the aorta. Cholesteryl acetate was chosen as an internal standard. In order to avoid interference of triglyceride and phospholipids
with the separation of the internal standard and 1,2-DG, a stepwise elution of lipids from the silicic acid column was used.
The development of Chromarods was done using two solvent systems and a three-step developing technique. Assay and recovery
of both 1,2-DG and cholesterol (as compared to cholesteryl acetate) were sufficient to measure changes in the 1,2-DG content
in blood vessels. After exposing the thoracic aorta to 10−5 M norepinephrine for 10 min, the 1,2-DG content increased nearly two-fold without significant change in cholesterol content. |
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