| 反相高效液相色谱-光电二极管阵列检测器法测定杜仲叶发酵液中绿原酸的含量 |
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| 引用本文： | 方传代,刘传安,邹盛勤.反相高效液相色谱-光电二极管阵列检测器法测定杜仲叶发酵液中绿原酸的含量[J].食品科学,2006,27(6):168-170. |
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| 作者姓名： | 方传代 刘传安 邹盛勤 |
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| 作者单位： | 1.School of Science, Yichun University, Yichun 336000, China;2.Bioengineering Research Institute of Yichun University, Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun 336000, China |
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| 摘 要： | 建立反相高效液相色谱(HPLC)法测定杜仲叶发酵液中绿原酸的含量。色谱条柱SymmetrySheildRP18柱(3.9×300mm,5μm),以水:甲醇:乙酸(88:12:1.2,V/V)为流动相,流速1.0ml/min,检测波长326nm,柱温25℃。绿原酸对照品在2.24～11.2μg范围内线性关系良好(r=0.9993)。本方法简便、准确,线性范围宽,可用于含绿原酸的原料及产品的质量控制。
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| 关 键 词： | 反相高效液相色谱法 杜仲叶 发酵液 绿原酸 |
| 文章编号： | 1002-6630（2006）06-0168-03 |
| 收稿时间： | 2005-08-28 |
| 修稿时间： | 2005-08-28 |
Determination of Chlorogenic Acid in Ferment Liquor of Eucommia ulmoides Oliv.Leaves by RP-HPLC-PAD |
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| FANG Chuan-dai,LIU Chuan-an,ZOU Sheng-qin.Determination of Chlorogenic Acid in Ferment Liquor of Eucommia ulmoides Oliv.Leaves by RP-HPLC-PAD[J].Food Science,2006,27(6):168-170. |
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| Authors: | FANG Chuan-dai LIU Chuan-an ZOU Sheng-qin |
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| Abstract: | A determination method of chlorogenic acid by high performance liquid chromtograph and photodiode array detector was established. The factors such as: the chromatographic column, SymmetrySheild RP18 (3.9×300mm, 5μm), the water- methanol-acetic acid (88:12:1.2, V/V) mobile phase with 1.0ml/min flow rate, the detected wavelength (326nm), and the column temperature(25℃) were adopted. The calibration curve of chlorogenic acid was linear under the content of 2.24 ～11.2μg, with the correlation coefficient 0.9993. The method is simple, accurate and of wider linear range. The method can be adapted to control the quality of material and products that contain chlorogenic acid. |
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| Keywords: | RP-HPLC Eucommia ulmoides Oliv leaves ferment liquor chlorogenic acid |
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