Minor Changes in Erythrocyte Osmotic Fragility in Trace Amine-Associated Receptor 5 (TAAR5) Knockout Mice |
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Authors: | Ilya S. Zhukov Larisa G. Kubarskaya Inessa V. Karpova Anastasia N. Vaganova Marina N. Karpenko Raul R. Gainetdinov |
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Affiliation: | 1.Institute of Translational Biomedicine, Saint Petersburg State University, 199034 Saint Petersburg, Russia; (I.S.Z.); (A.N.V.);2.Institute of Experimental Medicine, 197376 Saint Petersburg, Russia; (L.G.K.); (I.V.K.); (M.N.K.);3.Institute of Toxicology of Federal Medical-Biological Agency, 192019 Saint Petersburg, Russia;4.Saint Petersburg State University Hospital, Saint Petersburg State University, 199034 Saint Petersburg, Russia |
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Abstract: | Trace amine-associated receptors (TAARs) are a group of G protein-coupled receptors that are expressed in the olfactory epithelium, central nervous system, and periphery. TAAR family generally consists of nine types of receptors (TAAR1-9), which can detect biogenic amines. During the last 5 years, the TAAR5 receptor became one of the most intriguing receptors in this subfamily. Recent studies revealed that TAAR5 is involved not only in sensing socially relevant odors but also in the regulation of dopamine and serotonin transmission, emotional regulation, and adult neurogenesis by providing significant input from the olfactory system to the limbic brain areas. Such results indicate that future antagonistic TAAR5-based therapies may have high pharmacological potential in the field of neuropsychiatric disorders. TAAR5 is known to be expressed in leucocytes as well. To evaluate potential hematological side effects of such future treatments we analyzed several hematological parameters in mice lacking TAAR5. In these mutants, we observed minor but significant changes in the osmotic fragility test of erythrocytes and hematocrit levels. At the same time, analysis of other parameters including complete blood count and reticulocyte levels showed no significant alterations in TAAR5 knockout mice. Thus, TAAR5 gene knockout leads to minor negative changes in the erythropoiesis or eryptosis processes, and further research in that field is needed. The impact of TAAR5 deficiency on other hematological parameters seems minimal. Such negative, albeit minor, effects of TAAR5 deficiency should be taken into account during future TAAR5-based therapy development. |
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Keywords: | trace amines trace amine-associated receptor TAAR TAAR5 erythrocyte fragility hematology animal knockout model GPCR |
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