| 乙酰胆碱酯酶分子的原子力显微成像 |
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| 引用本文： | 张英鸽,王琛,赵德禄,白春礼,林樟,田芳,苏明.乙酰胆碱酯酶分子的原子力显微成像[J].电子显微学报,1999,18(1):94-98. |
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| 作者姓名： | 张英鸽 王琛 赵德禄 白春礼 林樟 田芳 苏明 |
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| 作者单位： | 1. 军事医学科学院毒物药物研究所,北京,100850
2. 中国科学院化学研究所,北京,100080 |
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| 摘 要： | 本文研究了用原子力显微技术对乙酰胆碱酯酶进行成像的方法。在新鲜裂解的云一表面镀一层金膜，金膜与疏基丙酸（ＭＰＡ）的疏基形成Ｓ－Ａｕ键。使ＭＰＡ的游离羧基再通过碳二亚胺反应与ＡＣｈＥ的碱性氨基酸的氨基形成肽键连接，ＡＣｈＥ因而被固定于支持物表面，这样固定的ＡＣｈＥ可获得清晰成像而不脱落。
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| 关 键 词： | 原子力显微技术 乙酰胆碱酯酶 蛋白质 |
Imaging of acetyl cholinesterase with atomic force microscopy |
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| ZHANG Ying-ge,WANG Chen,ZHAO De-lu,BAI Chun-li,LIN Zhang,TIAN Fang,SU Ming.Imaging of acetyl cholinesterase with atomic force microscopy[J].Journal of Chinese Electron Microscopy Society,1999,18(1):94-98. |
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| Authors: | ZHANG Ying-ge WANG Chen ZHAO De-lu BAI Chun-li LIN Zhang TIAN Fang SU Ming |
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| Abstract: | Method of imaging acetylcholinesterase (AChE) with atomic force microscopy has been studied.A gold film was evaporated onto the fresh surface of mica.By immersing the gold film in the mercaptopropionic acid (MPA) solution,Au S bond was formed between Au and the hydrosulfide group.The carboxyl group of MPA was activated by N ethyl N' (3 dimethylaminopropyl) carbodiimide (EDC) in the solution of EDC and then was allowed to react with AChE and thus peptide bond between the carboxyl group of MPA and the free amino group of the amino acid in AChE molecules.Clear image of AChE immobilized in this way could be obtained by atomic force microscopy. |
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| Keywords: | atomic force microscopy acetylcholinesterase protein |
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